Modifications of Ribosome Profiling that Provide New Data on the Translation Regulation. Biochemistry (Moscow), 86, №9, 1095-1106, 2021 DOI: 10.1134/S0006297921090054статья
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Дата последнего поиска статьи во внешних источниках: 23 сентября 2021 г.
Аннотация:Ribosome profiling (riboseq) has opened the possibilities for the genomewide studies of translation in all living organisms. This method is based on deep sequencing of mRNA fragments protected by the ribosomes from hydrolysis by ribonucleases, the socalled ribosomal footprints (RFPs). Ribosomal profiling together with RNA sequencing allows notonly to identify with a reasonable accuracy translated reading frames in the transcriptome, but also to track changes in gene expression in response to various stimuli. Notably, ribosomal profiling in its classical version has certain limitations. The size of the selected mRNA fragments is 2535 nts, while RFPs of other sizes are usually omitted from analysis. Also, ribosomal profiling “averages” the data from all ribosomes and does not allow to study specific ribosomal complexes associated with particular translation factors. However, recently developed modifications of ribosomal profiling provide answers to a number of questions. Thus, it has become possible to analyze not only elongating, but also scanning and reinitiating ribosomes,to study events associated with the collision of ribosomes during mRNA translation, to discover new ways of cotranslational assembly of multisubunit protein complexes during translation, and to selectively isolate ribosomal complexes associatedwith certain protein factors. New data obtained using these modified approaches provide a better understanding of the mechanisms of translation regulation and the functional roles of translational apparatus components.DOI