Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezersстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 сентября 2021 г.
Аннотация:Red blood cells (RBCs) are able to interact and communicate with endothelial cells (ECs). Undersome pathological or even normal conditions, the adhesion of RBCs to the endothelium can beobserved. Presently, the mechanisms and many aspects of the interaction between RBCs and ECsare not fully understood. In this work, we considered the interaction of single RBCs with singleECs in vitro aiming to quantitatively determine the force of this interaction using laser tweezers.Measurements were performed under di®erent concentrations of proaggregant macromoleculesand in the presence or absence of tumor necrosis factor (TNF-alpha) activating the ECs. We haveshown that the strength of interaction depends on the concentration of ¯brinogen or dextranproaggregant macromolecules in the environment. A nonlinear increase in the force of cellsinteraction (from 0.4 pN to 21 pN) was observed along with an increa se in the ¯brinogen con-centration (from 3 mg/mL to 9 mg/mL) in blood plasma, as well as with the addition of dextranmacromolecules (from 10 mg/mL to 60 mg/mL). Dextran with a higher molecular mass (500 kDa)enhances the adhesion of RBCs to ECs greater compared to the dextran with a lower molecularmass (70 kDa). With the preliminary activation of ECs with TNF--alpha, the force of interactionincreases. Also, the adhesion of echinocytes to EC compared to discocytes is signi¯cantly higher.These results may help to better understand the process of interaction between RBCs and ECs.