Recombinant Destabilase from the Medicinal leech: Preparation and Propertiesстатья

Информация о цитировании статьи получена из Scopus, Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 14 декабря 2016 г.

Работа с статьей

[1] Recombinant destabilase from the medicinal leech: Preparation and properties / A. S. Kurdyumov, V. A. Manuvera, D. N. Akhaev et al. // Russian Journal of Bioorganic Chemistry. — 2016. — Vol. 42, no. 1. — P. 42–52. Abstract—The procedure for obtaining an active recombinant destabilase from the medicinal leech in Escherichia coli cells was developed. The plasmids encoding an analogue of native destabilase, as well as the protein forms carrying polyhistidine sequence at the C and/or Nterminus of the polypeptide were obtained during the work. The producing strains of different forms of the protein were constructed, the cultivation pro cess was optimized. The conditions of renaturation of destabilase recombinant forms by dialysis and using chromatographic absorbent were selected. The muramidase activity towards cell walls of Micrococcus lyso deikticus bacferia and lytic activity towards E. coli were investigated. The dependence of pH and ionic strength of the solution on the activities was determined. The total antibacterial activity of destabilase towards E. coli was shown.

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