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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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The Influence of Linker’s Structure on the Performance of Novel HILIC Silica- and PS-DVB-based Stationary Phasesтезисы доклада Тезисы
- Авторы: Popov A., Chernobrovkina A., Zatirakha A., Smolenkov A., Shpigun O.
- Сборник: 48th International Symposium on High-Performance Liquid Phase Separations and Related Techniques
- Тезисы
- Год издания: 2019
- Место издания: University of Milano-Bicocca Milan
- Первая страница: 1
- Последняя страница: 2
- Аннотация: The majority of modern HILIC phases are based on silica substrate having limitedoperating pH stability. Hydrophobic substrates such as polystyrene-divinylbenzene (PSDVB)have always been considered non-prospective because of their low efficiency;however, its chemical stability in wide pH range provides advantages as compared tosilica substrate. The choice of an appropriate modification technique providing theattachment of hydrophilic functional layer effectively coating hydrophobic particles couldgive a possibility to use the prepared adsorbents in HILIC mode. Covalent attachment ofbranched hydrophilic polyamines such as polyethyleneimine (PEI) seems to be apromising strategy for this purpose.In our work we varied a distance between the hydrophilic functional layer andhydrophobic substrate surface for decreasing its negative influence on hydrophilicretention and efficiency. This strategy was realized by the attachment of the linkerscontaining one, two or three fragments of bifunctional 1,4-butanediol diglycidyl ether (1,4BDDGE) between PS-DVB and PEI layer, which was additionally hydrophilized withglycidol. To compare the effect of linker’s length and structure on the chromatographicproperties, a set of silica-based phases with the same linkers and functional layer wasalso synthesized.Chromatographic properties of the stationary phases were evaluated and compared usingTanaka test procedure and studying the separation of model mixtures of sugars and water-soluble vitamins in wide pH range varying the type and concentration of buffer solution inmobile phase.Hydrophilization technique used here demonstrated efficient coating of substrateparticles, confirmed by the insignificant difference in the retention factors for vitamins orcarbohydrates within the set of obtained phases based on the same substrate.Using longer and more branched linker in combination with a hydrophilic PEI layer made itpossible to increase the efficiency of PS-DVB-based adsorbent up to 40,000 theoreticalplates per meter for vitamins, which was comparable with the values for silica-based HILICphases. Thiamine, nicotinamide, pyridoxine, riboflavin, cyanocobalamin, ascorbic andnicotinic acids were separated on PEI-hydrophilized PS-DVB in gradient elution mode usingacetonitrile and 100mM ammonium formate buffer solution, pH 3.0. The obtained phaseswere successfully applied to the analyses of nutritional supplements.The proposed hydrophilization technique allowed us to prepare high performance PS-DVBbasedadsorbents for HILIC. Such approach can contribute to producing a new generationof highly stable hydrophilic stationary phases suitable for operating in the whole pHrange.
- Добавил в систему: Попов Александр Сергеевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | tezisyi.pdf | tezisyi.pdf | 287,2 КБ | 31 августа 2021 [Popov_Alexandr] |