Аннотация:The ATP amount and rate of ATP production by human mito-chondria are decreased in presence of Ab(1–42) peptide, which isknown that involved in pathogenesis of Alzheimer’s disease. The 1st experimental group of SH-SY5Y human neuroblastoma cellline were cultivated 24h (~90% confluence) with 200 nM Abmonomerased by standard procedure (Jao 1997, Dzinic 2018),2nd group (control) of cells had no additives, and the 3d group contained 0.1% DMSO (this amount of DMSO were added tothe 1st group with Ab). Mitochondria were isolated by standard procedure (Martin 1998, Daum 1982). To isolated mitochondria were added substrates of Complex I, II and IV and corresponded inhibitors of OxPhos Complexes – in separate experiments to highlight the changes of activity of each Complex induced byAb. Immediately before the measurements of luminescence to mitochondria were added ADP and luciferase-luciferin mixture(Ugarova et al. Patent RU 2420594, 2011). The luminescence(RLU) depending on time was plotted for each experimentalgroup. The maximum values of the first derivatives of the leftsides of the obtained bell shaped curves (the rise of the ATP production) were compared (the rate of ATP production). Also were compared the the peak heights (corresponded to the amount of the produced ATP). The presence of DMSO led to the slightlowering of ATP production by human mitochondria – amounton 9.3% and rate on 6.6% if compare with the control group(mean value for the Complexes I, II, IV). Cultivation of cell swith Ab makes mitochondria to produce 26.3% lower ATP (ifcompare with the group treated with DMSO) with 48.3% lowerrate. Widely used the methods which include the disruption ofcells or mitochondria to measure the total amount of ATP molecules in the lysates (Drew 2003, Gao 2009, Lim 2011). But wesuppose that the plotting the level of luminescence (ATP concentration) in time can give more information.
