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A study of the temperature dependence of tryptophan fluorescence lifetime in the range of –170°С to +20°С in various solventsстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 декабря 2021 г.
- Авторы: Paschenko V.Z., Gorokhov V.V., Korvatovsky B.N., Knox P.P., Grishanova N.P., Goryachev S.N.
- Журнал: Biophysics
- Том: 66
- Номер: 3
- Год издания: 2021
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 385
- Последняя страница: 394
- DOI: 10.1134/S0006350921030143
- Аннотация: The temperature dependences (–170°C...+20°C) of the fluorescence lifetime of tryptophan molecules in an aqueous solution, in solutions of glycerol (50 and 75% by volume), dimethyl sulfoxide (50 and 75% by volume) and in 1 M aqueous solution of trehalose were studied. In this temperature range, the fluorescence kinetics at room temperature in all samples were best approximated by three exponents with characteristic times of τ1 ~ 3 ns, τ2 ~ 4 ns, and τ3 ~ 15 ns. The temperature dependences of the fluorescence lifetime of the fastest and medium components in the temperature range from –60°C to 10°C were found to be directed in opposite directions (in antiphase). To explain these temperature dependences, a new model of the nature of multicomponent fluorescence was proposed, which considered the transition of the tryptophan molecule from the excited state to the charge-transfer state, the reverse transition to the excited state, as well as the radiative and nonradiative transitions of the charge-transfer state to the ground state. The results we obtained can serve as a basis for the interpretation of the experimental dependences of the lifetime of tryptophan fluorescence on the temperature in proteins.
- Добавил в систему: Нокс Петр Петрович