|
ИСТИНА |
Войти в систему Регистрация |
Интеллектуальная Система Тематического Исследования НАукометрических данных |
||
Hydrogen peroxide in tobacco stigma exudate affects pollen proteome and membrane potential in pollen tubesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 января 2022 г.
- Авторы: Breygina M., Klimenko E., Shilov E., Podolyan A., Mamaeva A., Zgoda V., Fesenko I.
- Журнал: Plant Biology
- Том: 23
- Номер: 4
- Год издания: 2021
- Издательство: Georg Thieme Verlag
- Местоположение издательства: Germany
- Первая страница: 592
- Последняя страница: 602
- Номер статьи: plb.13255
- DOI: 10.1111/plb.13255
- Аннотация: ROS are known to be accumulated in stigmas of different species and can possibly per-form different functions important for plant reproduction. Here we tested the assump-tion that one of their functions is to control membrane potential and provoke synthesis of unique proteins in germinating pollen.• We used spectrofluorometry and spectrophotometry to detect H2O2in stigma exudate,quantitative fluorescent microscopy of pollen tubes and flow cytometry of pollen pro-toplasts to reveal effects on membrane potential, and a label-free quantificationapproach to study pollen proteome changes after H2O2 treatment.• We found that in both growing pollen tubes and pollen protoplasts exudate causesplasmalemma hyperpolarization similar to that provoked by H2O2. This effect is abol-ished by catalase treatment and the ROS quencher, MnTMPP. Inhibitory analysis indi-cates probable participation of Ca2+- and K+-conducting channels in the observedhyperpolarization. For a deeper understanding of pollen response, we analysed pro-teome alterations in H2O2-treated pollen grains. We found 50 unique proteins and 20differently accumulated proteins that are mainly involved in cell metabolism, energet-ics, protein synthesis and folding.• Observed hyperpolarization and proteome alterations agree well with previouslyreported stimulation of pollen germination by H2O2 and sensitivity of Ca2+- and K+-conducting channels to this ROS. Thus, H2O2is one of the active substances in tobaccostigma exudate that stimulates various physiological processes in germinating pollen.
- Добавил в систему: Брейгина Мария Александровна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | plb.13255.pdf | 1,1 МБ | 16 мая 2021 [Breygina_Maria] |