Back to the future: optimised microwell culture of individual human preimplantation stage embryos

Gábor, V.; Parmegiani, L.; Machaty, Z.; Chen, W.B.; Sergey, Y.

Авторы: Gábor Vajta, Parmegiani Lodovico, Machaty Zoltan, Chen Wen Bin, Sergey Yakovenko
Журнал: Journal of Assisted Reproduction and Genetics
Том: 38
Номер: 10
Год издания: 2021
Издательство: Kluwer Academic/Plenum Publishers
Местоположение издательства: United States
Первая страница: 2563
Последняя страница: 2574
DOI: 10.1007/s10815-021-02167-4
Аннотация: Although in vitro culture of human embryos is a crucial step in assisted reproduction, the lack of focused research hampers worldwide standardisation and consistent outcomes. Only 1.2% of research papers published in five leading journals in humanreproduction in 2019 focused on in vitro culture conditions, creating the impression that the ptimisation process has approached its limits. On the other hand, in vitro culture of mammalian embryos is based on old principles, while there is no consensus on basic issues as density, time, medium change, gas atmosphere and small technical details including the way of drop preparation.This opinion paper aims to highlight and analyse the slow advancement in this field and stimulate research for simple and affordable solutions to meet the current requirements. A possible way for advancement is discussed in detail. Selection ofembryos with the highest developmental competence requires individual culture and modification of the widely used “drop under oil” approach. Current use of three-dimensional surfaces instead of large flat bottoms is restricted to time-lapse systems, but these wells are designed for optical clarity, not for the needs of embryos. The size and shape of the original microwells (Well of the Well; WOW) offer a practical and straightforward solution to combine the benefits of communal and individual incubation and improve the overall quality of cultured embryos.
Добавил в систему: Яковенко Сергей Александрович

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