Apoptotic Bodies: Selective Detection in Extracellular Vesicles

Авторы: Hauser Paul, Wang Sha, Didenko Vladimir V.
Сборник: Methods in Molecular Biology
Год издания: 2017
Издательство: Springer New York
Местоположение издательства: New York, N.Y., United States
Первая страница: 193
Последняя страница: 200
DOI: 10.1007/978-1-4939-6759-9_12
Аннотация: Normal and dying cells release various types of membrane-bound vesicles including microvesicles, exosomes, and apoptotic bodies. These vesicles play important roles in intercellular communication and signal transduction. However, their diverse forms and subtypes fluctuate in size and other properties. In result current purification approaches do not fully discriminate between different categories of extracellular vesicles. Here, we present a fluorescence technique that specifically identifies apoptotic bodies in preparations of microvesicles, exosomes, and other extracellular vesicles.The approach exclusively labels the vesicles that contain DNA with 5'PO4 blunt-ended DNA breaks, such as those produced by the apoptotic CAD nuclease during apoptotic DNA degradation. The technique can be useful in studies of apoptosis involving microvesicles and exosomes.
Добавил в систему: Didenko Vladimir Vladimirovich

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