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Comparison of quenching efficacy of SYBR Green I and PicoGreen fluorescence by ultrasmall gold nanoparticles in isotropic and liquid-crystalline DNA systemsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 февраля 2021 г.
- Авторы: Morozov V.N., Kolyvanova M.A., Dement'eva O.V., Rudoy V.M., Kuzmin V.A.
- Журнал: Journal of Molecular Liquids
- Том: 321
- Год издания: 2021
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Номер статьи: 114751
- DOI: 10.1016/j.molliq.2020.114751
- Аннотация: Gold nanoparticles (GNPs) are promising for sensors based on fluorescence quenching. Conjunction of GNPs to liquid crystalline (LC) matrix may improve their quenching properties. In the present work the quenching of SYBR Green I (SG) and PicoGreen (PG) fluorescence by negatively charged 2.5 nm GNPs was investigated in an isotropic DNA solution and in its cholesteric liquid crystalline dispersion (DNA CLCD). These dyes have similar chromophore structures and spectral properties but different charges: +2 (SG) and +3 (PG). The most efficient static fluorescence quenching was observed at the lowest concentration of dyes: in the DNA CLCD for SG (KSV = 7.4 × 107 M−1) and in the isotropic DNA solution for PG (KSV = 1.54 × 108 M−1). Since in the DNA solution quenching was due to the formation of a non-fluorescent complex with GNPs, the quenching was up to ~70-fold more efficient for PG than for SG. In the DNA CLCD the quenching efficacy of SG fluorescence increased up to ~15-fold because of the energy transfer. At the same time, due to steric hindrance for the interaction of PG with GNPs in the DNA CLCD caused by tight packaging of DNA molecules, the fluorescence quenching efficacy was ~5-fold higher in the isotropic solution.
- Добавил в систему: Дементьева Ольга Вадимовна
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