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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Hybrid Minimal Core Streptavidin-Obelin as a Versatile Reporter for Bioluminescence-based Bioassayстатья
- Авторы: Bashmakova Eugenia E., Krasitskaya Vasilisa V., Kudryavtsev Alexander N., Grigorenko Vitaly G., Frank Ludmila A.
- Журнал: Photochemistry and Photobiology
- Год издания: 2016
- Издательство: American Society for Photobiology
- Местоположение издательства: United States
- DOI: 10.1111/php.12648
- Аннотация: Ca2+ -regulated photoprotein obelin was genetically fused with a minimum-sized core streptavidin. Hybrid protein (SAV-OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid-phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV-OL has been shown to be a much more sensitive label than the chemical conjugate of a full-length streptavidin with obelin.
- Добавил в систему: Григоренко Виталий Георгиевич