Comparative analysis of genes encoding key steroid core oxidation enzymes in fast-growing Mycobacterium spp. Strains

Bragin, E.Y.; Shtratnikova, V.Y.; Dovbnya, D.V.; Schelkunov, M.I.; Pekov, Y.A.; Malakho, S.G.; Egorova, O.V.; Ivashina, T.V.; Sokolov, S.L.; Ashapkin, V.V.; Donova, M.V.

Авторы: Bragin E.Y., Shtratnikova V.Y., Dovbnya D.V., Schelkunov M.I., Pekov Y.A., Malakho S.G., Egorova O.V., Ivashina T.V., Sokolov S.L., Ashapkin V.V., Donova M.V.
Журнал: Journal of Steroid Biochemistry and Molecular Biology
Год издания: 2013
Издательство: Pergamon Press Ltd.
Местоположение издательства: United Kingdom
DOI: 10.1016/j.jsbmb.2013.02.016
Аннотация: A comparative genome analysis of Mycobacterium spp. VKM Ac-1815D, 1816D and 1817D strains used for efficient production of key steroid intermediates (androst-4-ene-3,17-dione, AD, androsta-1,4-diene-3,17-dione, ADD, 9Î±-hydroxy androst-4-ene-3,17-dione, 9-OH-AD) from phytosterol has been carried out by deep sequencing. The assembled contig sequences were analyzed for the presence putative genes of steroid catabolism pathways. Since 3-ketosteroid-9Î±-hydroxylases (KSH) and 3-ketosteroid-Î”1-dehydrogenase (Î”1 KSTD) play key role in steroid core oxidation, special attention was paid to the genes encoding these enzymes. At least three genes of Î”1 KSTD (kstD), five genes of KSH subunit A (kshA), and one gene of KSH subunit B of 3-ketosteroid-9Î±-hydroxylases (kshB) have been found in Mycobacterium sp. VKM Ac-1817D. Strains of Mycobacterium spp. VKM Ac-1815D and 1816D were found to possess at least one kstD, one kshB and two kshA genes. The assembled genome sequence of Mycobacterium sp. VKM Ac-1817D differ from those of 1815D and 1816D strains, whereas these last two are nearly identical, differing by 13 single nucleotide substitutions (SNPs). One of these SNPs is located in the coding region of a kstD gene and corresponds to an amino acid substitution Lys (135) in 1816D for Ser (135) in 1815D. The findings may be useful for targeted genetic engineering of the biocatalysts for biotechnological application.
Добавил в систему: Пеков Юрий Алексеевич

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Comparative analysis of genes encoding key steroid core oxidation enzymes in fast-growing Mycobacterium spp. Strainsстатья

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