Assessment of Fibrinogen Macromolecules Interaction with Red Blood Cells Membrane by Means of Laser Aggregometry, Flow Cytometry, and Optical Tweezers Combined with Microfluidicsстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 25 ноября 2020 г.
Аннотация:An elevated concentration of fibrinogen in blood is a significant risk factor during many
pathological diseases, as it leads to an increase in red blood cells (RBC) aggregation, resulting in
hemorheological disorders. Despite the biomedical importance, the mechanisms of fibrinogeninduced
RBC aggregation are still debatable. One of the discussed models is the non‐specific
adsorption of fibrinogen macromolecules onto the RBC membrane, leading to the cells bridging in
aggregates. However, recent works point to the specific character of the interaction between
fibrinogen and the RBC membrane. Fibrinogen is the major physiological ligand of glycoproteins
receptors IIbIIIa (GPIIbIIIa or αIIββ3 or CD41/CD61). Inhibitors of GPIIbIIIa are widely used in
clinics for the treatment of various cardiovascular diseases as antiplatelets agents preventing the
platelets’ aggregation. However, the effects of GPIIbIIIa inhibition on RBC aggregation are not
sufficiently well studied. The objective of the present work was the complex multimodal in vitro
study of the interaction between fibrinogen and the RBC membrane, revealing the role of GPIIbIIIa
in the specificity of binding of fibrinogen by the RBC membrane and its involvement in the cells’
aggregation process. We demonstrate that GPIIbIIIa inhibition leads to a significant decrease in the
adsorption of fibrinogen macromolecules onto the membrane, resulting in the reduction of RBC aggregation. We show that the mechanisms underlying these effects are governed by a decrease in
the bridging components of RBC aggregation forces.