The role of blood plasma porphyrins in the effects of He-Ne laser on human leukocytesстатья

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Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.

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[1] The role of blood plasma porphyrins in the effects of he-ne laser on human leukocytes / G. I. Klebanov, T. V. Chichuk, A. N. Osipov, Y. A. Vladimirov // Biophysics. — 2005. — Vol. 50, no. 4. — P. 627–631. He-Ne-laser-induced effects in human blood leukocytes in the presence of autologous plasma were investigated. Experiments were performed in two ways: (1) laser irradiation of cells in the presence of plasma or (2) irradiation and subsequent addition of plasma to the cell suspension. The concentration dependences of plasma additions were evaluated. To obtain different concentrations of porphyrins in plasma samples, we either diluted the samples with PBS or selected patients with different plasma porphyrin content. The effects of laser irradiation were characterized by the maximally effective dose (Dmax) of irradiation and the degree of maximal cell activation (Amax, priming index). In the first series of experiments, we irradiated leukocytes in autologous plasma from patients with pneumonia and bromchial asthma. It was found that Dmax decreased with increasing porphyrin concentration in plasma. It was observed that, at low porphyrin concentrations, Amax increased severalfold with increasing photosensitizer concentration, but then decreased as the porphyrin content exceeded 0.46 pmol. The same effects were revealed at high doses of laser irradiation. Very similar effects were found in experiments with the addition of irradiated plasma to cells. However, the Amax value was considerably less than that after irradiation in the presence of plasma (160% vs.230-270% upon combined irradiation of cells and plasma). The Dmax value was higher in the series of experiments in which plasma was irradiated separately. The results suggest that laser-induced leukocyte activation can be mediated by blood plasma porphyrins and the products of lipid peroxidation formed as a result of porphyrin-photosensitized lipid oxidation.

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