Similarities and differences between 6S RNAs from Bradyrhizobium japonicum and Sinorhizobium meliloti

Burenina, O.Y.; Elkina, D.A.; Migur, M.Y.; Oretskaya, T.S.; Evguenieva-Hackenberg, E.; Hartmann, R.K.; Kubareva, E.A.

Авторы: Burenina O.Y., Elkina D.A., Migur M.Y., Oretskaya T.S., Evguenieva-Hackenberg E., Hartmann R.K., Kubareva E.A.
Журнал: Journal of Microbiology
Том: 58
Год издания: 2020
Издательство: Han-Gug Misaengmul Hag-hoe/The Microbiological Society of Korea
Местоположение издательства: Korea, Republic of
Первая страница: 945
Последняя страница: 956
DOI: 10.1007/s12275-020-0283-1
Аннотация: 6S RNA, a conserved and abundant small non-coding RNA found in most bacteria, regulates gene expression by inhibiting RNA polymerase (RNAP) holoenzyme. 6S RNAs from α-proteobacteria have been studied poorly so far. Here, wepresent a first in-depth analysis of 6S RNAs from two α-proteobacteria species, Bradyrhizobium japonicum and Sinorhizobium meliloti. Although both belong to the order Rhizobiales and are typical nitrogen-fixing symbionts of legumes,their 6S RNA expression profiles were found to differ: B. japonicum 6S RNA accumulated in the stationary phase, thus being reminiscent of Escherichia coli 6S RNA, whereas S. meliloti6S RNA level peaked at the transition to the stationary phase, similarly to Rhodobacter sphaeroides 6S RNA. We demonstratedin vitro that both RNAs have hallmarks of 6SRNAs: they bind to the σ70-type RNAP holoenzyme and serve as templates for de novo transcription of so-called product RNAs (pRNAs) ranging in length from ~13 to 24 nucleotides, with further evidence of the synthesis of even longer pRNAs.Likewise, stably bound pRNAs were found to rearrange the 6S RNA structure to induce its dissociation from RNAP. Compared with B. japonicum 6S RNA, considerable conformational heterogeneity was observed for S. meliloti 6S RNA and its complexes with pRNAs, even though the two 6S RNAsshare ~75% sequence identity. Overall, our findings suggest that the two rhizobial 6S RNAs have diverged with respect to their regulatory impact on gene expression throughout thebacterial life cycle.
Добавил в систему: Кубарева Елена Александровна

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