Bacteriolytic activity of human blood plasmaserotransferrin

Levashov, P.A.; Matolygina, D.A.; Ovchinnikova, E.D.; Ushakova, D.A.; Burmakin, V.V.; Cherdyntseva, T.A.; Eremeev, N.L.; Tishkov, V.I.; Levashov, A.V.

Авторы: Levashov P.A., Matolygina D.A., Ovchinnikova E.D., Ushakova D.A., Burmakin V.V., Cherdyntseva T.A., Eremeev N.L., Tishkov V.I., Levashov A.V.
Сборник: FEBS Open Bio
Серия: Suppl. 1
Том: 9
Тезисы
Год издания: 2019
Место издания: John Wiley & Sons Inc United States Poland United States
Первая страница: 72
Последняя страница: 72
DOI: 10.1002/2211-5463.12675
Аннотация: In the current work, bacteriolytic activity of serotransferrin wasdiscovered. Although transferrins have been known for a longtime, some of their functions are not completely clear. There isthe information that transferrins can suppress the bacterialgrowth, however, bacteriolytic activity of these proteins has notbeen described before. In our experiments, human blood plasmaproteins were separated to identify and study new bacteriolyticfactors. It was found that the protein fraction with molecularweights between 60 and 80 kDa showed bacteriolytic activity onbacteria cells. After a few stages of chromatography the activeprotein was puriﬁed. Trypsinolysis of the isolated protein fol-lowed by mass spectrometry analysis allowed us to identify thisprotein as a serotransferrin. Puriﬁed serotransferrin demonstratesbacteriolytic activity against Escherichia coli, Micrococcus luteus,Bacillus subtilis and Bacillus megaterium cells. pH proﬁles ofactivity are characterized at a maximum pH of 8.7–8.9. At anoptimal pH value (8.8), the rates of lysis of various bacteria inthe presence of serotransferrin and lysozyme were compared,considering the rate of lysis as a percentage of “lysozyme activityagainst E. coli”. Lysozyme at a concentration of 0.3 lg/mL actseffectively against E. coli (100%) and M. luteus (68%), less efﬁ-ciently against B. subtilis (18%) and practically does not act atall against B. megaterium (1%). Serotransferrin at a concentra-tion of 3 lg/mL acts effectively against E. coli (61%), less efﬁ-ciently against M. luteus and B. subtilis (13% and 12%), butrelatively efﬁciently against B. megaterium (23%). The detailedinvestigation of serotransferrin bacteriolytic properties can beuseful in drug-design to ﬁght antibiotic-resistant pathogenicmicroorganisms. The authors thank Dr Marina Serebryakova,leading researcher of the Belozersky Institute of Physico-Chemi-cal Biology, for conducting a mass spectrometric study.
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