Four New Genes of Cyanobacterium Synechococcus elongatus PCC 7942 Are Responsible for Sensitivity to 2-Nonanone

Koksharova, O.A.; Popova, A.A.; Plyuta, V.A.; Khmel, I.A.

Авторы: Koksharova O.A., Popova A.A., Plyuta V.A., Khmel I.A.
Журнал: Microorganisms
Том: 8
Номер: 8
Год издания: 2020
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1234
Последняя страница: 1234
DOI: 10.3390/microorganisms8081234
Аннотация: Microbial volatile organic compounds (VOCs) are cell metabolites that affect many physiological functions of prokaryotic and eukaryotic organisms. Earlier we have demonstrated the inhibitory effects of soil bacteria volatiles, including ketones, on cyanobacteria. Cyanobacteria are very sensitive to the ketone action. To investigate the possible molecular mechanisms of ketone 2-nonanone influence on cyanobacterium Synechococcus sp. PCC 7942 cells we applied a genetic approach. After Tn5-692 transposon mutagenesis performance the several 2-nonanone resistant mutants have been selected. Four different mutant strains were used for identification of the impaired genes (Synpcc7942_1362, Synpcc7942_0351, Synpcc7942_0732, Synpcc7942_0726) that encode correspondingly: 1) a murein-peptide ligase Mpl that is involved in the biogenesis of cyanobacteria cell wall; 2) a putative ABC transport system substrate-binding proteins MlaD, which participates in ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane by aberrantly localized phospholipids transport from outer to inner membranes of bacterial cells; 3) a conserved hypothetical protein that is encoding by gene belonging to phage gene cluster in Synechococcus genome; 4) a protein containing the VRR-NUC (virus-type replication-repair nuclease) domain present in restriction-modification enzymes involved in replication and DNA repair. Obtained results demonstrated that 2-nonanone may have different targets in Synechococcus sp. PCC 7942 cells. Among them are proteins involved in biogenesis and functioning of the cyanobacteria cell wall and protein participating in stress response at DNA restriction-modification level. This paper is the first report about the genes that encoding protein products, which can be affected by 2-nonanone.Keywords: microorganisms; genetic control; 2-nonanone; VOCs; ABC-transporter; cell membranes; resistant mutants; transposon mutagenesis; DNA restriction-modification; ketone toxicity
Добавил в систему: Кокшарова Ольга Алексеевна

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Four New Genes of Cyanobacterium Synechococcus elongatus PCC 7942 Are Responsible for Sensitivity to 2-Nonanoneстатья

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