Copy Number Variation of Human Satellite III (1q12) With Aging

Ershova, E.S.; Malinovskaya, E.M.; Konkova, M.S.; Veiko, R.V.; Umriukhin, P.E.; Martynov, A.V.; Kutsev, S.I.; Veiko, N.N.; Kostyuk, S.V.

Авторы: Ershova Elizaveta S., Malinovskaya Elena M., Konkova Marina S., Veiko Roman V., Umriukhin Pavel E., Martynov Andrey V., Kutsev Sergey I., Veiko Natalia N., Kostyuk Svetlana V.
Журнал: Frontiers in genetics
Том: 10
Год издания: 2019
Издательство: Frontiers Research Foundation.
Местоположение издательства: Lausanne, Switzerland
DOI: 10.3389/fgene.2019.00704
Аннотация: Human satellite DNA is organized in long arrays in peri/centromeric heterochromatin. There is little information about satellite copy number variants (CNVs) in aging and replicative cell senescence (RS). Materials and Methods: Biotinylated pUC1.77 probe was used for the satellite III (f-SatIII) quantitation in leukocyte DNA by the non-radioactive quantitative hybridization for 557 subjects between 2 and 91 years old. The effect of RS and genotoxic stress (GS, 4 or 6 µM of K2CrO4) on the f-SatIII CNV was studied on the cultured human skin fibroblast (HSF) lines of five subjects. Results: f-SatIII in leukocyte and HSFs varies between 5.7 and 40 pg/ng of DNA. During RS, the f-SatIII content in HSFs increased. During GS, HSFs may increase or decrease f-SatIII content. Cells with low f-SatIII content have the greatest proliferative potential. F-SatIII CNVs in different individuals belonging to the different generations depend on year of their birth. Children (born in 2005-2015 years) differed significantly from the other age groups by low content and low coefficient of variation of f-SatIII. In the individuals born in 1912-1925 and living in unfavorable social conditions (FWW, the Revolution and the Russian Civil War, SWW), there is a significant disproportion in the content of f-SatIII. The coefficient of variation reaches the maximum values than in individuals born in the period from 1926 to 1975. In the group of people born in 1990-2000 (Chernobyl disaster, the collapse of the Soviet Union, and a sharp decline in the population living standard), again, there is a significant disproportion of individuals in the content of f-SatIII. A similar disproportion was observed in the analysis of a group of individuals born in 1926-1975 who in their youth worked for a long time in high-radioactive environment. Conclusion: In generations that were born and who lived in childhood in a period of severe social perturbations or in conditions of environmental pollution, we found a significant increase in leukocyte DNA f-SatIII variability. It is hypothesized that the change of the f-SatIII content in the blood cells reflects the body response to stress of different nature and intensity.
Добавил в систему: Костюк Светлана Викторовна

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

Copy Number Variation of Human Satellite III (1q12) With Agingстатья

Прикрепленные файлы