Growth and biotin synthesis of Sporobolomyces pararoseus and Rhizopus delemar under different substrate ratiosстатья
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Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
Аннотация:Biotin synthesis by the yeast Sporobolomyces pararoseus and the microscopic fungus Rhizopus delemar was studied in batch cultures at different substrate ratios in the medium. The overall (exocellular + endocellular) biotin content in the yeast was 4.5-6.0 micrograms per 1 g of biomass on different media and did not depend on the growth phase. The content of biotin in the fungus was 7.5-9.0 micrograms per 1 g of biomass and depended neither on the growth phase nor on the quantitative ratio between components of the growth medium. In contrast to the yeast, the content of exocellular biotin per 1 g of the mycelium increased with the fungal growth, and the yield of biotin was maximal (25-29 micrograms/g) at the stationary growth phase. The maximal rate of biotin synthesis by the both organisms coincided in time with their maximal growth rate.