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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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A Comparative study of the kinetic parameters and the secondary structure of two staphylolytic enzymesстатья
- Авторы: Filatova L., Donovan D., Klyachko N.
- Журнал: Annali d’Italia
- Том: 1
- Номер: 5
- Год издания: 2020
- Первая страница: 10
- Последняя страница: 16
- Аннотация: Lysins LysK and K-L are capable of lysing cells of antibiotic–resistant strains of Staphylococcus aureus and can be considered as promising antibacterial agents. The chimeric lysin K-L was created on the basis of the LysK enzyme. In order to successfully use the enzymes in practice it is important to study their kinetic parameters under various conditions and to find the relationship “secondary structure-stability”. Optimal conditions for the manifestation of activity of the lysins LysK and K-L were determined (temperature, pH, NaCl concentration). At a storage temperature (4°C), physiological pH level (7.5) and various NaCl concentrations (10–1000 mM), the lysins have high half-inactivation times, with the maximum values of 310 days for K-L, 380 days for LysK. For the lysin LysK, the half–inactivation time and the secondary structure do not change with a change in the concentration of NaCl. For the lysin K–L an increase in the concentration of NaCl causes disordering in the secondary structure and a change in the half–inactivation time. Under the conditions of functioning (37ºС, 150 mM NaCl, pH 7.5), the lysins are characterized by high half–inactivation times (10–15 hours). At 42.5°C, there is a sharp decrease in the stability of the lysins caused by denaturation. Research results can be used in the development of antibacterial drugs.
- Добавил в систему: Филатова Любовь Юрьевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Annali_dItalia_No5_2020_part_1-10-16.pdf | 538,7 КБ | 23 марта 2020 [FilatovaLYu] |