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Patch-clamp-spectroscopy of ROS effects and role of the redox-patchclamp-spectroscopy in the aging and age-related disease diagnosticsтезисы доклада
- Автор: Gradov O.V.
- Сборник: International Conference “Biomembranes 2016: Mechanisms of Aging and Age-related Diseases” [Book of Abstracts]
- Тезисы
- Год издания: 2016
- Место издания: MIPT
- Первая страница: 98
- Аннотация: It has been known for a long time that reactive oxygen species are chemical agents of aging [1-4] based on ROS-induced DNA damage [5], tissue chondriome damage [3,6,7] (and, consequently, respiratory function destroy [8]) and membrane lipid peroxidation [9]. Well known, that membrane electrophysiology regulated by redox-signaling and, consequently, by ROS. Good method for study of ROS effect with synchronal correlation analysis of ROS content in a biological media is patch-clamp or voltage-clamp [10-12]. This has led several authors to propose the use of patch-clamp spectroscopy [13,14] as a method for analyzing of membrane effects of ROS and one of powerful instruments for searching of biochemical descriptors of aging and some age-related diseases. LITERATURE: [1] Blagosklonny M.V. Aging: ROS or TOR // Cell Cycle. 2008. 7(21): 3344-3354. [2] Napoli E. et al. Frataxin, iron-sulfur clusters, heme, ROS, and aging // Antiox. Redox Signal. 2006. 8(3-4): 506-516. [3] Ross C. Folate, mitochondria, ROS, and the aging brain // AJM. 2005. 118(10):1174. [4] Fernandez-Marcos P.J., Nóbrega-Pereira S. NADPH: new oxygen for the ROS theory of aging // Oncotarget. 2016. (in press). [5] Guachalla L.M., Rudolph K.L. ROS induced DNA damage and checkpoint responses: influences on aging? // Cell Cycle. 2010. 9(20):4058-4060. [6] Plohnke N. et al. Proteomic analysis of mitochondria from senescent P. anserina casts new light on ROS-dependent aging mechanisms // Exp. Gerontol. 2014. 56:13-25. [7] Nasto L.A. et al. Mitochondrial-derived reactive oxygen species (ROS) play a causal role in agingrelated intervertebral disc degeneration // JOR. 2013. 31(7):1150-1157. [8] Genova M.L., Lenaz G. The Interplay Between Respiratory Supercomplexes and ROS in Aging // Antiox. Redox Signal. 2015. 23(3): 208-238. [9] Leutner S. et al. ROS generation, lipid peroxidation and antioxidant enzyme activities in the aging brain // J. Neur. Trans. 200. 108(8-9):955-967. [10] Lukyanetz E.A., Shkryl V.M. Scientific and technological aspects of oxygen-sensitive electrodes for measurements of oxygenpartial pressure in patch-clamp experiments // J. Biochem. Biophys. Meth. 2003. 55(1):37-52. [11] Jamieson C.V., MacDonald A.G. A method of patch clamp recording in hyperbaric oxygen // Undersea Hyperb. Med. 2002. 29(4):286-293. [12] Cerbai E. et al. Cellular electrophysiological basis for oxygen radical-induced arrhyth-mias // Circulation. 1991. 84(4):1773-1782. [13] Gradov O.V. [«Multifactor patch-clamp spectroscopy» as a method for analysis of the cell signalling and function regulation via the ion channels] // Tsitologiia. 2015. 57(9):625-626 [Article in Russian] [14] Gradov O.V. [Patch-clamp-spectroscopy as a potential diagnostic method for molecular oncology and analysis of ion channels as a possible molecular targets] // Usp. Mol. Oncol. 2015. 2(4): 66 [Article in Russian]
- Добавил в систему: Gradov Oleg V.