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Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cellsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 декабря 2016 г.
- Авторы: Dugina Vera, Alieva Irina, Khromova Natalya, Kireev Igor, Gunning Peter, Kopnin Pavel
- Журнал: Oncotarget
- Том: 5
- Номер: 0
- Год издания: 2016
- Издательство: Impact Journals
- Местоположение издательства: Albany, N.Y., United States
- Первая страница: 1
- Последняя страница: 17
- DOI: 10.18632/oncotarget.12236
- Аннотация: // Vera Dugina1,3, Irina Alieva1,3, Natalya Khromova2, Igor Kireev1, Peter W. Gunning3 and Pavel Kopnin2 1 Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia 2 Blokhin Russian Cancer Research Center, Moscow, Russia 3 School of Medical Science, The University of New South Wales, NSW, Sydney, Australia Correspondence to: Pavel Kopnin, email: // Keywords: EB1, +TIPs, microtubules, actin isoforms, β-actin, γ-actin Received: September 9, 2016 Accepted: September 18, 2016 Published: September 24, 2016 Abstract Actin microfilaments and microtubules are both highly dynamic cytoskeleton components implicated in a wide range of intracellular processes as well as cell-cell and cell-substrate interactions. The interactions of actin filaments with the microtubule system play an important role in the assembly and maintenance of 3D cell structure. Here we demonstrate that cytoplasmic actins are differentially distributed in relation to the microtubule system. LSM, 3D-SIM, proximity ligation assay (PLA) and co-immunoprecipitation methods applied in combination with selective depletion of β- or γ-cytoplasmic actins revealed a selective interaction between microtubules and γ-, but not β-cytoplasmic actin via the microtubule +TIPs protein EB1. EB1-positive comet distribution analysis and quantification have shown more effective microtubule growth in the absence of β-actin. Our data represent the first demonstration that microtubule +TIPs protein EB1 interacts mainly with γ-cytoplasmic actin in epithelial cells.
- Добавил в систему: Дугина Вера Борисовна