[Phosphorylation and other properties of proteins binding single-stranded DNA (SSB-proteins) from chromatin and extrachromatin fractions of Ehrlich ascites carcinoma]

Авторы: Koterov A.N., Novoradovskaia N.A., Pushkareva N.B., Vorotnikov A.V., Nikol'skiĭ A.V., Risnik V.V.
Журнал: Biokhimii∎a (Moscow, Russia)
Том: 56
Номер: 4
Год издания: 1991
Издательство: IZDATELSTVO NAUKA
Местоположение издательства: LENINGRADSKOE OTDELENIE MAKAROVA D4, STPETERSBURG, RUSSIA
Первая страница: 666
Последняя страница: 673
Аннотация: A comparative study of single-stranded DNA-binding proteins (SSB-proteins) isolated from chromatin and the extrachromatin fraction of Ehrlich ascites tumour cells was carried out. No differences were found either in SDS-gel electrophoretic mobility or in the single-stranded DNA-binding capacity and stimulation of the replicative synthesis of DNA. However, chromatin SSB-proteins contained 1.4-1.5 times more phosphate than extrachromatin proteins. Both preparations could be phosphorylated in vitro by protein kinase C and cAMP-dependent protein kinase, but the chromatin proteins were phosphorylated in a lesser degree. In parallel with phosphorylation the SSB-proteins displayed a higher binding affinity for ssDNA-cellulose. Phosphorylation can thus be regarded as a means of regulation of the SSB-protein function, in particular, their interaction with chromatin DNA.
Добавил в систему: Воротников Александр Вячеславович

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