Reactivation of dormant and nonculturable bacterial forms from paleosoils and subsoil permafrostстатья
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Дата последнего поиска статьи во внешних источниках: 13 февраля 2018 г.
Аннотация:Methods of reactivating the dormant forms (DFs) and nonculturable cells (NCs) of the bacterial
communities of buried paleosoils and subsoil permafrost stored for long periods of time (thousands to millions
of years), including completely sterile samples (CFU = 0), were developed. They were based on washing the
DFs and NCs to remove anabiosis autoinducers (spore germination autoinhibitors) and introducing low molec-
ular weight extracellular growth regulators of microbial or plant origin, such as alkylhydroxybenzenes of the
alkylresorcinol subtype, indoleacetic acid, and wheat germ agglutinin. It was revealed that the dormant com-
munities of permafrost and buried soils differed in their sensitivity to reactivating factors, probably due to dif-
ferent natural storage conditions of the tested soil substrates and the heterogeneity of dormant populations. The
latter was confirmed by FISH (fluorescent in situ hybridization): applying the reactivation methods to the cells
of the dormant permafrost community resulted in an increase in the number of metabolically active cells from
5 to 77% of their total number. In contrast, the addition of microbial anabiosis autoinducers (C12-AHB) to back-
ground surface soil and permafrost samples caused the transition of bacterial cells to the dormant or the non-
culturable state, depending on the C12-AHB concentration and the sensitivity of the cells from the control soil
or permafrost 'to it. The data obtained contribute to our knowledge concerning the role of intercellular commu-
nication factors and the survival of microorganisms under extreme environmental conditions.
Keywords: survival of bacteria, buried soils, permafrost subsoil deposits, dormant cells, nonculturable cells,
intercellular regulators, FISH