A  bicistronic plasmid encoding brain-derived  neurotrophic  factor and urokinase  plasminogen  activatorstimulates peripheral nerve regeneration after injury

Karagyaur, M.; Rostovtseva, A.; Semina, E.; Klimovich, P.; Balabanyan, V.; Makarevich, P.; Popov, V.; Stambolsky, D.; Tkachuk, V.

Авторы: Karagyaur M., Rostovtseva A., Semina E., Klimovich P., Balabanyan V., Makarevich P., Popov V., Stambolsky D., Tkachuk V.
Журнал: Journal of Pharmacology and Experimental Therapeutics
Том: 372
Номер: 3
Год издания: 2020
Издательство: American Society for Pharmacology and Experimental Therapeutics
Местоположение издательства: United States
Первая страница: 248
Последняя страница: 255
DOI: 10.1124/jpet.119.261594
Аннотация: Timely nerve restoration is an important factor for the successful regeneration of tissues and organs. It is known that axon regeneration following nerve injury is a multifactorial process that depends on the local expression of neurotrophins, including brain-derived neurotrophic factor (BDNF). Along with the survival of neurons, the active reorganization of the extracellular matrix is an important step for the growth of axons to their targets. Urokinase serine protease is part of the plasminogen activator system, which provides the vectoriality of the process of fibrinolysis and matrix reorganization, facilitating the growth of nerves to their targets. Based on this and in view of the results of our previous studies, we suggest that a combined bicistronic plasmid encoding the complementary proteins BDNF and urokinase may be beneficial in nerve regeneration. The ability of this bicistronic plasmid to stimulate nerve restoration was confirmed by in vitro stimulation of Neuro2a neurite growth and in vivo nerve conductivity and histology studies. To our knowledge, this is the first article that demonstrates the effectiveness of a bicistronic plasmid containing the human genes BDNF and uPA in the regeneration of the injured peripheral nerve. The results obtained demonstrate that plasmid vectors encoding several complementary-active therapeutic proteins may serve as a basis for developing prospective treatments for a wide range of multicomponent neural system disorders, such as nerve trauma.
Добавил в систему: Карагяур Максим Николаевич

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

A bicistronic plasmid encoding brain-derived neurotrophic factor and urokinase plasminogen activatorstimulates peripheral nerve regeneration after injuryстатья

Прикрепленные файлы