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Anti-stress activity of Propionibacterium freudenreichii: identification of a reactivative proteinстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Vorobjeva L., Leverrier P., Zinchenko A., Boyaval P., Khodjaev E., Varioukhina S., Ponomareva G., Gordeeva E., Jan G.
- Журнал: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
- Том: 85
- Номер: 1
- Год издания: 2004
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 53
- Последняя страница: 62
- DOI: 10.1023/B:ANTO.0000020276.18127.99
- Аннотация: Propionibacterium freudenreichii subsp. shermanii is known to prevent mutations caused by various agents such as N-methyl-N'-nitro-N-nitrosoguanidine, 9-aminoacridine, 4-nitro-quinoline-1-oxide and by UV radiation in both prokaryotic and eukaryotic cells. It was also shown to prevent or repair damage caused by H2O2 or UV radiation in Salmonella typhimurium and Escherichia coli, a characteristic previously designated as reactivative effect. In order to characterise this effect at the molecular level, we have purified the active component from a P. freudenreichii cell-free extract using a combination of ammonium sulfate precipitation, anion-exchange and size-exclusion chromatography. The isolated 35 kDa protein was then identified using both N-terminal and internal peptide sequencing as a cysteine synthase. The latter was localised in the P. freudenreichii proteomic map. It is constitutively expressed but also clearly induced during adaptation to detergent and heat, but not acid, stresses. The biological meaning of cysteine synthase in the context of adaptation to oxidative and non-oxidative stresses is discussed.
- Добавил в систему: Воробьева Лена Ивановна