Аннотация:Chitosan is known to be a good absorbent for many organic compounds; in particular, a large number of studies have been devoted to the chemosorption of quinones on chitosan due to the practical importance of this process, e.g. synthesis of new polymers and purification of wastewaters from phenolic compounds after their oxidation. The most extensive studies on the subject were carried out by Paine et al [1], Muzarreli et al [2] and some other groups. It has been supposed that this process proceeds as Michaelis addition [3]. However, despite a variety of possible applications of this process to our best knowledge we were the first researches to propose the application of such reaction for the purposes of analytical chemistry.
The aim of this material is to present the data of a cycle of studies carried out by the authors devoted to utilizing chitosan both as an analytical reagent and enzyme immobilization matrix in the same system. Such advantageous combination provided the creation of a simple and reliable analytical device for laboratory and in-field analysis which can operate in aqueous or mixed media and determine some phenolic compounds.