Biochemical evidence for intertypic genetic recombination of poliovirusesстатья

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[1] Biochemical evidence for intertypic genetic recombination of polioviruses / L. I. Romanova, E. A. Tolskaya, M. S. Kolesnikova, V. I. Agol // FEBS Letters. — 1980. — Vol. 118, no. 1. — P. 109–112. Volume 118, number 1 FEBS LETTERS August 1980 BIOCHEMICAL EVIDENCE FOR INTERTYPIC GENETIC RECOMBINATION OF POLIOVIRUSES L. I. ROMANOVA, E. A. TOLSKAYA, M. S. KOLESNIKOVA and V. I. AGOL Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region 142782 and Moscow State University, Moscow I1 7234, USSR Received 3 July 1980 1. Introduction Two basically different kinds of genetic recombina- tion are known to occur in RNA-containing viruses. Firstly, there is a high-frequency recombination among viruses with segmented RNA genomes, such as reo- viruses [ 1 J or orthomyxoviruses [2]. This type of genetic recombination is due to physical exchange of separate RNA molecules (‘segments’) between paren- tal genomes and, therefore, may be designated ‘genetic reassortment’ [3]. Secondly, relatively low-frequency recombination is reported to exist in picornaviruses, whose genome is represented by a single RNA mole- cule [4,5]. The nature of the latter type of recombi- nation is poorly understood, but it is believed to be true inheritance in a single RNA molecule of genetic properties of both parents [4,5]. Evidence for this interpretation has only been provided by purely gene- tic techniques and direct biochemical proof that recombinants do acquire genetic information from more than one parent is lacking. Experimental solu- tion of this problem became more feasible when recombinants between different poliovirus serotypes were obtained (E. A. T., M. S. K., in preparation). Well-established differences between the genomes of different types of polioviruses ([6-lo], L. I. R., E. A. T., V. I. A., submitted) make possible a direct comparison of recombinants with each of their parents. Here we report results showing that some poly- peptides coded for by the genome of a type l/type 3 poliovirus recombinant are indistinguishable from the corresponding polypeptides of the type 1 parent, whereas some other recombinant-specific polypeptides appear to be of the type 3 origin. These results are the first direct biochemical evidence for intermolec- ular recombination between RNA genomes. [ DOI ]

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