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Translational barrier in central region of encephalomyocarditis virus genome. Modulation by elongation factor 2 (eEF-2)статья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Svitkin Y.V., Agol V.I.
- Журнал: European journal of biochemistry / FEBS
- Том: 133
- Номер: 1
- Год издания: 1983
- Издательство: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
- Местоположение издательства: Oxford, UK, England
- Первая страница: 145
- Последняя страница: 154
- Аннотация: A fractionated cell-free system from Krebs-2 cells was prepared which contained ribosomes and a high-speed supernatant. When this system was programmed with encephalomyocarditis virus RNA, the synthesis of a precursor of capsid proteins, polypeptide preA, proceeded at a rate not very different from that observed in unfractionated extracts, whereas the synthesis of more distally encoded proteins, in particular polypeptide F, was greatly retarded, if not abolished. A protein was purified from the cytoplasmic extracts of Krebs-2 cells which greatly enhanced production of polypeptide F as well as other noncapsid proteins in the fractionated system. By several criteria, this protein was identified as eukaryotic elongation factor 2 (eEF-2). By using the ADP-ribosylation assay, it was found that the fractionated system contained about 15% of the amount of eEF-2 present in the unfractionated extracts. The results suggest that changes in the eEF-2 content may affect the elongation rate differently at different regions of the RNA template.
- Добавил в систему: Агол Вадим Израилевич