MYCOBACTERIUM VACCAE LYSATE INDUCES IL-10 RESPONSE IN HUMAN IMMUNE CELLS

Nesmiyanov, P.P.; Strygin, A.V.; Tolkachev, B.E.; Morkovin, E.I.; Strygina, A.O.; Gutov, M.V.

Авторы: Nesmiyanov P.P., Strygin A.V., Tolkachev B.E., Morkovin E.I., Strygina A.O., Gutov M.V.
Сборник: Вторая Московская конференция по молекулярной аллергологии
Тезисы
Год издания: 2019
Первая страница: 49
Последняя страница: 49
Аннотация: Background: M. vaccae lysate used in topical emollient have been shown to prevent atopic dermatitis in high-risk infants. However, the mechanisms of action of lysate is not fully understood. Methods: We characterized consequences of immune responses to sonic lysate of M. vaccae strain DSM 43292 (MVSL43292) in a model system containing whole human blood samples from 32 healthy donors, using commercially available B. longum, B. bifidum and B. adolescentis lysate (BL) as a control and PBS as a negative control. The innate and adaptive cytokine responses were evaluated in cell-free supernatants of the whole blood incubations: IL-1β, IL-6, TNFα, IL-8, IL-10, IL-2, IL-4, IL-13, IL-12p70, and IFNγ. Results: Exposure to MVSL43292 or BL resulted in the initiation of immune responses and change of secreted cytokines profile. Similar levels were recorded for IL-1β, IL-6, TNFα, IL-8, IL-2, IL-12p70, and IFNγ. However, median IL-4, 13 levels were diminished in MVSL43292-incubated blood (1.67 (2.39) pg/mL vs 3.86 (4.05) pg/mL (p=0.003); 5.44 (6.98) pg/mL vs 9.38 (8.22) pg/mL (p=0.0023), respectively) and IL-10 level was higher (7.03 (7.14) pg/mL vs 1.11 (0.96) pg/mL (p=0.0012)) compared to blood incubated with BL. Data are presented as median (IQR), Bonferroni-adjusted α-level was applied for multiple comparisons. Discussion: Atopic diseases are on the rise on urbanized societies. One explanation for this is ‘hygiene hypothesis’ claiming that reduced exposure to environmental microorganisms leads to Th2-skewed immune cells differentiation and the consequent clinical presentations. One of the microorganisms responsible for the proper immune system development is M. vaccae. We have proposed recently its lysate use in topical formulations for the primary prevention of atopic dermatitis. Here we showed that mechanism of action of M. vaccae lysate is associated with induction of anti-inflammatory cytokine profile. Further research is needed to elucidate whether these events are responsible for the anti-allergic MVSL43292 effects in early life and whether MVSL43292 or its analogues can be used for the treatment of atopic dermatitis and other allergic diseases.
Добавил в систему: Несмиянов Павел Павлович

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

MYCOBACTERIUM VACCAE LYSATE INDUCES IL-10 RESPONSE IN HUMAN IMMUNE CELLSтезисы доклада Тезисы

Прикрепленные файлы