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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Nestin-GFP Transgene Reveals Neural Precursor Cells in Adult Skeletal Muscleстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Birbrair Alexander, Wang Zhong-Min, Messi Maria Laura, Enikolopov Grigori N., Delbono Osvaldo
- Журнал: PLoS ONE
- Том: 6
- Номер: 2
- Год издания: 2011
- Издательство: Public Library of Science
- Местоположение издательства: United States
- DOI: 10.1371/journal.pone.0016816
- Аннотация: Background: Therapy for neural lesions or degenerative diseases relies mainly on finding transplantable active precursor cells. Identifying them in peripheral tissues accessible for biopsy, outside the central nervous system, would circumvent the serious immunological and ethical concerns impeding cell therapy. Methodology/Principal Findings: In this study, we isolated neural progenitor cells in cultured adult skeletal muscle from transgenic mice in which nestin regulatory elements control GFP expression. These cells also expressed the early neural marker Tuj1 and light and heavy neurofilament but not S100 beta, indicating that they express typical neural but not Schwann cell markers. GFP+/Tuj1+ cells were also negative for the endothelial and pericyte markers CD31 and alpha-smooth muscle actin, respectively. We established their a) functional response to glutamate in patch-clamp recordings; b) interstitial mesenchymal origin; c) replicative capacity; and d) the environment necessary for their survival after fluorescence-activated cell sorting. Conclusions/Significance: We propose that the decline in nestin-GFP expression in muscle progenitor cells and its persistence in neural precursor cells in muscle cultures provide an invaluable tool for isolating a population of predifferentiated neural cells with therapeutic potential.
- Добавил в систему: Ениколопов Григорий Николаевич