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Viral and transgenic reporters and genetic analysis of adult neurogenesisстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Enikolopov G., Overstreet-Wadiche L., Ge S.
- Журнал: Cold Spring Harbor perspectives in biology
- Том: 7
- Номер: 8
- Год издания: 2015
- Издательство: Cold Spring Harbor Laboratory Press
- Местоположение издательства: United States
- DOI: 10.1101/cshperspect.a018804
- Аннотация: Stem and progenitor cells of the developing and adult brain can be effectively identified and manipulated using reporter genes, introduced into transgenic reporter mouse lines or recombinant viruses. Such reporters rely on an ever-increasing variety of fluorescent proteins and a continuously expanding list of regulatory elements and of mouse lines engineered for cell- or time-specific recombination. An important extension of stem-cell-based genetic strategies is an opportunity to explore the properties of newly generated neurons and their contribution to synaptic plasticity. Here, we review available strategies for marking and quantifying various classes of stem and progenitor cells in the adult brain, genetically tracing their progeny, and studying the properties of stem cells and new neurons. We compare various experimental approaches to labeling and investigating stem cells and their progenyand discuss caveats and limitations inherent to each approach. © 2015, Cold Spring Harbor Laboratory Press; all rights reserved.
- Добавил в систему: Ениколопов Григорий Николаевич