POLARIZATION FLOUROIMMUNOASSAY FOR RAPID, SPECIFIC DETECTION OF PESTICIDES: EMERGING TECHNOLOGIES

Автор: EREMIN SA
Сборник: IMMUNOANALYSIS OF AGROCHEMICALS: EMERGING TECHNOLOGIES Book Series: ACS SYMPOSIUM
Серия: ACS SYMPOSIUM
Том: 586
Год издания: 1995
Место издания: ACS
Первая страница: 223
Последняя страница: 234
Аннотация: Polarization fluoroimmunoassay (PFIA) is a simple, inexpensive, easily automated screening method for pesticide residues in large numbers of environmental samples. PFIA measures the increased polarization of fluorescence when a fluorophore-labeled hapten (tracer) is bound by specific antibody, and the decreased signal when free analyte competes with the tracer for binding. No separation of free and bound analyte is required. PFIAs for 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2.4,5-T), simazine, and atrazine were automated on an Abbott TDx Analyzer. Ten water samples of 0.05 mt can be analyzed in 7 minutes, with detection limits of 100 ng/mL for 2,4-D and 5 ng/mL for simazine, and coefficients of variation < 5%.
Добавил в систему: Еремин Сергей Александрович

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