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One-step label-free chemiluminescent assay for determination of exonuclease III activity towards hairpin oligonucleotidesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 декабря 2019 г.
- Авторы: Burkin K.M., Bodulev O.L., Gribas A.V., Sakharov I.Yu
- Журнал: Enzyme and Microbial Technology
- Том: 131
- Год издания: 2019
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 109419
- DOI: 10.1016/j.enzmictec.2019.109419
- Аннотация: Fast label-free chemiluminescent assay for determination of exonuclease III (ExoIII) activity measured towards hairpin oligonucleotide substrates was developed. The designed substrates consisted of EAD2 aptamer to hemin which was associated with DNA sequence complementary to 5’-terminus fragment of EAD2. In the presence of ExoIII the associated sequence of the hairpin stem was digested, producing EAD2 aptamer which reacted with hemin with the formation of peroxidase-mimicking DNAzyme (PMDNAzyme). The catalytic activity of the produced PMDNAzyme was measured towards luminol/H2O2. Under the optimized conditions the limit of detection and sensitivity of the one-step chemiluminescent assay of ExoIII were 7.3 nM and 1.7×108 M−1, respectively. The coefficient of variation (CV) was lower than 6%.
- Добавил в систему: Сахаров Иван Юрьевич