Determination of fluoride, cyanide, and thiocyanate using horseradish peroxidase immobilized on modified silica gelстатья

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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.

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[1] Determination of fluoride, cyanide, and thiocyanate using horseradish peroxidase immobilized on modified silica gel / K. V. Yablotskiy, O. V. Radhul, I. A. Veselova, T. N. Shekhovtsova // Analytical Letters. — 2007. — Vol. 40, no. 8. — P. 1521–1539. Silica gels modified with different functional groups (amino, epoxy, cycloepoxy, isocyanate, and thiocyanate) were used for the covalent immobilization of horseradish peroxidase (HRP). The catalytic activity and stability of the obtained enzyme preparations were studied using the reaction of o-dianisidine oxidation with hydrogen peroxide as an indicator. The covalent immobilization of horseradish peroxidase using silica gel modified with thiocyanate groups provided not only the improvement of the enzyme stability, but also the development of the sensitive, rapid, and simple procedures for the determination of fluoride, cyanide, and thiocyanate. Enzymatic determination of inorganic anions is based on their inhibitory effect on the enzyme as the ligands capable to form stable complexes with Fe(III)-HRP cofactor. The proposed procedures were applied for the determination of F- in mineral and drinking waters; CN- and SCN- - in biological fluids (blood and saliva). Copyright © Taylor & Francis Group, LLC. [ DOI ]

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