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A peroxidase-based method for the determination of dopamine, adrenaline, and α-methyldopa in the presence of thyroid hormones in pharmaceutical formsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Poliakov A.E., Dumshakova A.V., Muginova S.V., Shekhovtsova T.N.
- Журнал: Talanta
- Том: 84
- Номер: 3
- Год издания: 2011
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 710
- Последняя страница: 716
- DOI: 10.1016/j.talanta.2011.01.074
- Аннотация: The analytical properties of two commercial plant peroxidases isolated from horseradish roots and soybean hulls in the catalysis of the transformation of some catecholamines were demonstrated in the absence and presence of thyroid hormones (l-thyroxine and 3,3′,5′-iodothyronine). For the first time the reactions of dopamine, adrenaline, and α-methyldopa oxidation with H2O2 catalysed by horseradish peroxidase with the addition of l-thyroxine as the amplification agent were studied and proposed as the indicator reactions for the simple and rapid enzymatic determination of the indicated catecholamines in their concentration ranges 0.5-300, 4-300, and 100-400 μM, respectively. The catalytic activity of the enzyme (characterized by the reaction rate) was controlled spectrophotometrically. The optimum conditions for the indicator reactions were thoroughly characterized. The mechanism of the stimulatory effect of l-thyroxine on the oxidation of the catecholamines was discussed. The developed enzymatic procedures were successfully applied for the determination of dopamine, adrenaline, and α-methyldopa in some pharmaceutical forms. © 2011 Elsevier B.V. All rights reserved. All rights reserved.
- Добавил в систему: Шеховцова Татьяна Николаевна