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ω-Lsp-IA, a novel modulator of P-type Ca2+ channelsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 июня 2016 г.
- Авторы: Pluzhnikov Kirill, Vassilevski Alexander, Korolkova Yuliya, Fisyunov Alexander, Iegorova Olena, Krishtal Oleg, Grishin Eugene
- Журнал: Toxicon : official journal of the International Society on Toxinology
- Том: 50
- Номер: 7
- Год издания: 2007
- Издательство: Pergamon Press
- Местоположение издательства: United States
- Первая страница: 993
- Последняя страница: 1004
- DOI: 10.1016/j.toxicon.2007.07.004
- Аннотация: A novel polypeptide, designated ω-Lsp-IA, which modulates P-type Ca2+ channels, was purified from the venom of the spider Geolycosa sp. ω-Lsp-IA contains 47 amino acid residues and 4 intramolecular disulfide bridges. It belongs to a group of spider toxins affecting Ca2+ channels and presumably forms the inhibitor cystine knot (ICK) fold. Peculiar structural features (a cluster of positively charged residues in the C-terminal loop of the peptide and a regular distribution of hydrophobic residues) that may play a decisive role in the ω-Lsp-IA mechanism of action were located. Recombinant ω-Lsp-IA was produced in prokaryotic expression system and was shown to be structurally and functionally identical to the native toxin. At saturating concentration (10 nM), the peptide clearly slows down the activation kinetics and partially inhibits the amplitude of P-current in rat cerebellar Purkinje neurons. Prominent deceleration of the activation kinetics is manifested as the appearance of a five-fold slower component of the current activation. The specificity of action of ω-Lsp-IA on different Ca2+ channel types was studied in isolated hippocampal neurons of rat. ω-Agatoxin IVA completely removed the effect of ω-Lsp-IA on the whole-cell Ca2+ current. Therefore, ω-Lsp-IA appears to act specifically on P-type Ca2+ channels.
- Добавил в систему: Василевский Александр Александрович