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Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilizationстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Alcântara-Neto Agostinho, Fernandez-Rufete Marina, Corbin Emilie, Tsikis Guillaume, Uzbekov Rustem, Garanina Anastasia, Coy Pilar, Almiñana Carmen, Mermillod Pascal
- Журнал: Reproduction, Fertility and Development
- Год издания: 2019
- Издательство: Commonwealth Scientific and Industrial Research Organization Publishing
- Местоположение издательства: Australia
- DOI: 10.1071/RD19058
- Аннотация: High polyspermy incidence is one of the major limitations of porcine in vitro fertilization (IVF). The addition of oviductal fluid (OF) during IVF reduces polyspermy without decreasing fertilization rate. Since extracellular vesicles (EVs) have been described as important OF components, our aim was to evaluate the effect of porcine oviductal EVs (poEVs) on IVF efficiency in comparison with porcine OF (fresh and lyophilized). OF was collected from abattoir oviducts by PBS flush and poEVs were isolated by serial ultracentrifugation. Four IVF treatments were performed: poEVs (0.2mg/mL); OF (10%); Lyophilized/reconstituted pure OF (LOF; 1%) and without supplementation (Control). Penetration, monospermy and IVF efficiency were evaluated. Transmission electron microscopy showed an EVs population mainly composed of exosomes (83%; 30-150nm). The poEVs supplementation during IVF increased monospermy (44%) compared to control (17%), while maintaining an acceptable penetration rate (61% vs.78%, respectively) in a similar way to OF/LOF. Western blotting revealed poEVs proteins involved in early reproductive events, including zona pellucida hardening. In conclusion, our findings showed that poEVs are porcine OF key components and may play roles in porcine fertilization and polyspermy regulation, suggesting that poEVs supplementation is a reliable strategy to decrease porcine polyspermy and improve in vitro embryo production outcomes.
- Добавил в систему: Узбеков Рустем Эдуардович