Carbon-based nanomaterials as a support for peptides and proteins tritium labelling

Badun, G.A.; Chernysheva, M.G.; Bunyaev, V.A.; Ksenofonov, A.L.

Авторы: Badun G.A., Chernysheva M.G., Bunyaev V.A., Ksenofonov A.L.
Сборник: Book of Abstract 14th International Conference "Advanced Carbon Nanostructures" - ACNS'2019, July 1-5, 2019 St. Petersburg, Russia
Тезисы
Год издания: 2019
Место издания: Ioffe Physico-Technical Institute St. Petersburg
Первая страница: 299
Последняя страница: 299
Аннотация: Tritium thermal activation method is a tool for radiolabeling different organic compounds from amino acids [1] to humic substances [2] and carbon-based nanomaterials [3]. The method is based on the short-exposure bombardment of solid compound with tritium atoms that a generated on the surface of tungsten filament at 1700 – 2000 K. The compound is usually adsorbed on the walls of glass reaction flask. However, the application of other substrates can influence on the result of tritium interaction with organic molecules. In present work we have studied the influence of carbon-based materials (activated carbon, graphene oxide, reduced graphene oxide, nanotubes or nanodiamonds) on the specific radioactivity and intramolecular distribution of tritium in oligopeptide (Dalargin) and bovine serum albumin (BSA). It was shown that deposition of dalargin of carbon-based substrates significantly changes tritium distribution in the amino acids residues comparing with thick target on the glass surface. When tritium atoms interact with dalargin on the carbon surface the significant increase in the phenylalanine radioactivity indicating that the isotopic exchange occurs by electrophilic mechanism as well as by radical mechanism. Noted that changes in tritium content in glycine, leucine and tyrosine result from different in the structure of the adsorption layers on the carbon surfaces. In the case of BSA the changes in the intramolecular tritium distribution was not so significant. At the same time, a pronounced effect of increasing the specific radioactivity of the protein was observed comparing with the experiment on the glass surface under the similar conditions. The increase in the specific radioactivity of protein by an order of magnitude is explained by higher availability of protein on carbon surface to the interaction with atomic tritium. The effect of changes of tritium atoms reaction ability on the carbon surface is not evident because of large size of protein globule. This work was supported by Russian Foundation for Basic Research (grants № 18-33-20147, 17-03-00985). References A. Badun, M.G. 1. Chernysheva, A.L. Ksenofontov // Radiochimica Acta (2012) 100, 401. 2. A. Badun, M.G. Chernysheva, Z.A. Tyasto, N.A. Kulikova, A.V. Kudryavtsev, I.V. Perminova // Radiochimica Acta (2010), 98, 161. 3. A. Badun, M.G. Chernysheva, A.V. Grigorieva, E.A. Eremina, A.V. Egorov. // Radiochimica Acta (2016), 104, 593.
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