Sizes of Bacterial Cells in Soils Determined by Cascade Filtration Techniqueстатья

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Дата последнего поиска статьи во внешних источниках: 9 июня 2016 г.

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[1] Polyanskaya L. M., Gorodnichev R. B., Zvyagintsev D. G. Sizes of bacterial cells in soils determined by cascade filtration technique // Biology Bulletin. — 2013. — Vol. 40, no. 2. — P. 130–137. traditional method and the cascade filtration technique. The total number of bacteria in these soils, which was obtained in filters of different diameters during filtering the suspension of a certain amount, is 1.5–5 times higher than that obtained by the traditional method. In the structure of the bacterial biomass in both soils, the biomass of bacterial cells with a diameter of 0.38–0.43 μm was dominating by 8–90%. In the typical cher nozem, the biomass of cells with a diameter of 0.17 μm was slightly more than 1%; in the mountainmeadow soil, the percentage of the biomass of cells with a diameter of 0.17 μm increased by 5%. The average volume and diameter of the bacteria in the studied soils were calculated. In typical chernozem, the average volume of bacterial cells was equal to 0.0046 μm3 and the diameter was 0.206 μm. In the mountainmeadow soils, these values were slightly lower, 0.0038 μm3 and 0.194 μm, respectively. The biomass of the bacterial cells, which is usually calculated based on the cell volume of 0.1 μm3, is overestimated by about five times when counting the number on the filters. The percentage of the real biomass of soil bacteria is traditionally much lower than that estimated. [ DOI ]

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