Coprecipitation of proteins and DNA-protein complexes with magnesium sarcolysate: a study of an association of topoisomerase I and RNA polymerase with DNAстатья
Дата последнего поиска статьи во внешних источниках: 3 октября 2016 г.
Аннотация:A specific cosedimentation of proteins and their complexes with DNA at low temperature (M-band technique) has been demonstrated. Model experiments with reconstituted SV40 DNA-topoisomerase I and SV40 DNA-E. coli RNA polymerase complexes demonstrated the potential and capacities of the method. It allows fractionation of DNA-protein complexes from naked DNA and has greater range, higher reproducibility and lower background values than similar methods.