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Evidence for the presence of a new NAD+-dependent formate dehydrogenase in Pseudomonas sp.101 cells grown on a molybdenum-containing mediumстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
- Авторы: Karzanov V.V., Bogatsky Yu A., Tishkov V.I., Egorov A.M.
- Журнал: FEMS Microbiology Letters
- Том: 51
- Номер: 1
- Год издания: 1989
- Издательство: Blackwell
- Местоположение издательства: United Kingdom
- Первая страница: 197
- Последняя страница: 200
- Аннотация: The facultatively methylotrophic bacterium Pseudomonas sp. 101, grown on methanol in presence of molybdate, contains a new formate dehydrogenase (N-FDH) catalyzing NAD+-dependent oxidation of formate. The activity of this N-FDH could also be measured in presence of artificial electron acceptors, ferricyanide and 2,6-dichlorophenol indophenol. This new enzyme is absent in cells grown on a methanol-containing medium with tungstate, where only another two, previously described formate dehydrogenases, which are active only with NAD+ or only with artificial acceptors, respectively, were determined. The N-FDH was partially purified by a combination of ion-exchange and gel-filtration chromatography, and was shown to differ in its properties from the known NAD+-dependent counterpart.
- Добавил в систему: Тишков Владимир Иванович