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Foot and mouth disease virus polyepitope protein produced in bacteria and plants induces protective immunity in guinea pigsстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 октября 2013 г.
- Авторы: Andrianova E.P., Krementsugskaia S.R., Lugovskaia N.N., Mayorova T.K., Borisov V.V., Eldarov M.A., Ravin N.V., Folimonov A.S., Skryabin K.G.
- Журнал: Biochemistry (Moscow)
- Том: 76
- Номер: 3
- Год издания: 2011
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 339
- Последняя страница: 346
- Аннотация: The goal of this project was to develop an alternative foot and mouth disease (FMD) vaccine candidate based on a recombinant protein consisting of efficient viral epitopes. A recombinant gene was designed that encodes B-cell epitopes of proteins VP1 and VP4 and T-cell epitopes of proteins 2C and 3D. The polyepitope protein (H-PE) was produced in E. coli bacteria or in N. benthamiana plants using a phytovirus expression system. The methods of extraction and purification of H-PE proteins from bacteria and plants were developed. Immunization of guinea pigs with the purified H-PE proteins induced an efficient immune response against foot and mouth disease virus (FMDV) serotype O/Taiwan/99 and protection against the disease. The polyepitope protein H-PE can be used as a basis for developing a new recombinant vaccine against FMD.
- Добавил в систему: Эльдаров Михаил Анатольевич