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The RING for gypsy moth control: Topical application of fragment of its nuclear polyhedrosis virus anti-apoptosis gene as insecticideстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 апреля 2017 г.
- Авторы: Oberemok V.V., Laikova K.V., Zaitsev A.S., Gushchin V.A., Skorokhod O.A.
- Журнал: Pesticide Biochemistry and Physiology
- Том: 131
- Год издания: 2016
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 32
- Последняя страница: 39
- DOI: 10.1016/j.pestbp.2016.01.006
- Аннотация: Numerous studies suggest a cellular origin for the Lymantria dispar multicapsid nuclear polyhedrosis virus (LdMNPV) anti-apoptosis gene IAPs, thus opening a possibility to use the fragments of these genes for modulation of host metabolism. We report here the strong insecticidal and metabolic effect of single-stranded antisense DNA fragment from RING (really interesting new gene) domain of gypsy moth LdMNPV IAP-3 gene: specifically, on reduction of biomass (by 35%) and survival of L. dispar caterpillars. The treatment with this DNA fragment leads to a significantly higher mortality rates of female insects (1.7 fold) accompanied with the signs of apoptosis. Additionally, we show increased expression of host IAP-1, caspase-4 and gelsolin genes in eggs laid by survived females treated with RING DNA fragments accompanied with calcium and magnesium imbalance, indicating that the strong stress reactions and metabolic effects are not confined to treated insects but likely led to apoptosis in eggs too. The proposed new approach for insect pest management, which can be considered as advancement of “microbial pesticides”, is based on the application of the specific virus DNA, exploiting the knowledge about virus–pest interactions and putting it to the benefit of mankind.
- Добавил в систему: Гущин Владимир Алексеевич