Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germlineстатья

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[1] Repeat-associated sirnas cause chromatin silencing of retrotransposons in the drosophila melanogaster germline / M. S. Klenov, S. A. Lavrov, A. D. Stolyarenko et al. // Nucleic Acids Research. — 2007. — Vol. 35, no. 16. — P. 5430–8. Silencing of genomic repeats, including transposable elements, in Drosophila melanogaster is mediated by repeat-associated short interfering RNAs (rasiRNAs) interacting with proteins of the Piwi subfamily. rasiRNA-based silencing is thought to be mechanistically distinct from both the RNA interference and microRNA pathways. We show that the amount of rasiRNAs of a wide range of retroelements is drastically reduced in ovaries and testes of flies carrying a mutation in the spn-E gene. To address the mechanism of rasiRNA-dependent silencing of retrotransposons, we monitored their chromatin state in ovaries and somatic tissues. This revealed that the spn-E mutation causes chromatin opening of retroelements in ovaries, resulting in an increase in histone H3 K4 dimethylation and a decrease in histone H3 K9 di/trimethylation. The strongest chromatin changes have been detected for telomeric HeT-A elements that correlates with the most dramatic increase of their transcript level, compared to other mobile elements. The spn-E mutation also causes depletion of HP1 content in the chromatin of transposable elements, especially along HeT-A arrays. We also show that mutations in the genes controlling the rasiRNA pathway cause no derepression of the same retrotransposons in somatic tissues. Our results provide evidence that germinal Piwi-associated short RNAs induce chromatin modifications of their targets.From Duplicate 1 ( Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline - Klenov, Mikhail S; Lavrov, Sergey a; Stolyarenko, Anastasia D; Ryazansky, Sergey S; Aravin, Alexei a; Tuschl, Thomas; Gvozdev, Vladimir A )From Duplicate 2 ( Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline - Klenov, M S; Lavrov, S A; Stolyarenko, A D; Ryazansky, S S; Aravin, A A; Tuschl, T; Gvozdev, V A )DA - 200709140 (Chromatin)0 (Drosophila Proteins)0 (RNA, Small Interfering)0 (Retroelements)EC 3.6.1.- (Adenosine Triphosphatases)EC 3.6.1.- (spindle E protein, Drosophila)SB - IM. [ DOI ]

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