Аннотация:A one-step synthesis of a conducting copolymer of aniline and 2-aminophenethyl alcohol has been performed on a poly (2-acrylamido-2-methylpropanesulfonic acid) matrix (PAMPS). Highly redox-potential laccase from the fungus Trametes hirsuta was served as a catalyst for polymerization and hydroxybenzotriazole (HBT) was used as a mediator. As a result the conductive copolymer was obtained, the primary alcohol groups of which were oxidized to aldehyde groups. The formation of carbonyl groups in the copolymer chain was confirmed by a silver mirror test and FTIR studies. Peaks at 1740 and 1207-1220 cm-1 were observed on the FTIR spectrum of the copolymer, which can be attributed to tensile and deformation vibrations of the carbonyl group.
For the modification of the copolymer laccase substrate L-dioxyphenylalanine (DOPA) was used. Since the optimum pH of T. hirsuta laccase activity is 4.5 and Schiff bases are unstable in acidic medium the forming copolymer/DOPA conjugate was reduced with sodium borohydride. As a result of laccase-catalyzed oxidation of DOPA the product with an absorption maximum in the 570 nm area was formed in the conjugate.
Thus, the possibility of using the laccase-mediator system for the synthesis of the conducting copolymer of aniline and 2-aminophenethyl alcohol with reactive aldehyde groups was demonstrated. The formation of aldehyde groups in the copolymer chain allows further modification of the resulting product in order to obtain conducting composites that can be useful for biosensor devices, neural interfaces, etc.
