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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Stimulatory and Toxic Effects of Neurotransmitters on the lux Operon-Dependent Bioluminescence of Escherichia coli K12 TGIстатья
Дата последнего поиска статьи во внешних источниках: 27 сентября 2019 г.
- Авторы: Sorokina E.V., Vodolazov I.R., Oleskin A.V.
- Журнал: Journal of Pharmacy and Nutrition Sciences
- Том: 9
- Номер: 3
- Год издания: 2019
- Первая страница: 136
- Последняя страница: 143
- Аннотация: Abstract: Background: The normal functioning of the brain requires neuromediators, i.e., substances that transmitmessages between nervous cells. Neurochemicals also function as signals that are involved in communication amongthe microorganisms that inhabit the human organism. While the impact of “classical” neurotransmitters includingcatecholamines, serotonin, and histamine on microorganisms has been investigated in a number of recent publications,this work provides evidence for the stimulatory and inhibitory (toxic) effects of some other important neurochemicals thathave not received sufficient attention in the literature.Methods: The biosensor was based on a GM Escherichia coli K12 strain (TGI) that contained the lux operon of theluminescent soil bacterium Photorhabdus luminescencens ZMI. The biosensor was exposed to the action of the testedneurotransmitters for 15 mins to 144 hrs. The intensity of bacterial luminescence (counts / second) was monitored in thecontrol and the experimental samples with an 1251 BioOrbit luminometer (Finland).Results: Neurochemicals such as putrescine, acetylcholine, taurin, and indole were found to stimulate, at lowconcentrations (0.1-10 μM), the luminescence of the strain E. coli K12 TGI containing the lux operon from Photorhabdusluminescencens ZMI. At higher concentrations, putrescine, taurin, and indole exerted a weak toxic influence, i.e. theymarginally attenuated the luminescence of E. coli K12 TGI.Conclusions: Based on the data obtained, a regulatory, presumably receptor-dependent, effect is exerted by the testedneurochemicals on the bacterium E. coli K12 TGI, in an analogy to their impact on nervous, immune, and otherspecialized types of eukaryotic cells. However, high neurochemical concentrations are likely to produce nonspecificeffects on the bacterial luciferase system and/or on membrane phosphorylation.Keywords: Biotesting, bacterial luminescence, toxicity, neurochemicals, biogenic amines, amino acids.
- Добавил в систему: Сорокина Елена Владимировна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | JPANSV9N3A1-Oleskin_1.pdf | JPANSV9N3A1-Oleskin_1.pdf | 546,3 КБ | 10 ноября 2019 [Alexander_Oleskin] | |
| 2. | Полный текст | JPANSV9N3A1-Oleskin_1_E2kPUYs.pdf | 546,3 КБ | 10 ноября 2019 [Alexander_Oleskin] |