Characterization of the enhancer element of the Danio rerio minor globin gene locusстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 6 мая 2016 г.
Аннотация:In Danio rerio, the alpha- and beta-globin
genes are present in two clusters: a major cluster located on
chromosome 3 and a minor cluster located on chromosome
12. In contrast to the segregated alpha- and beta-globin
gene domains of warm-blooded animals, in Danio rerio,
each cluster contains both alpha- and beta-globin genes.
Expression of globin genes present in the major cluster is
controlled by an erythroid-specific enhancer similar to the
major regulatory element of mammalian and avian alphaglobin
gene domains. The enhancer controlling expression
of the globin genes present in the minor locus has
not been identified yet. Based on the distribution of epigenetic
marks, we have selected two genomic regions that
might harbor an enhancer of the minor locus. Using transient
transfection of constructs with a reporter gene, we
have demonstrated that a ~500-bp DNA fragment located
~1.7 Kb upstream of the αe4 gene possesses an erythroidspecific
enhancer active with respect to promoters present
in both the major and the minor globin gene loci of Danio
rerio. The identified enhancer element harbors clustered
binding sites for GATA-1, NF-E2, and EKLF similar to the
enhancer of the major globin locus on chromosome 3. Both
enhancers appear to have emerged as a result of independent
evolution of a duplicated regulatory element present
in an ancestral single alpha-/beta-globin locus that existed
before teleost-specific genome duplication.