P.7.003 Acute hypoxia, modelled in early pregnancy, causes transgenerational effects on child neurodevelopmentстатья Тезисы

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Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.

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[1] P.7.003 acute hypoxia, modelled in early pregnancy, causes transgenerational effects on child neurodevelopment / A. V. Graf, T. Y. Dunaeva, L. K. Trofimova et al. // European Neuropsychopharmacology. — 2011. — Vol. 21, no. Supplement 2. — P. 170. Introduction.: Prenatal stress is one of the main reasons of the phenomenon of intrauterine programming, which shows its effects even in adulthood. It is well proved, that negative influence of different pathologic factors during pregnancy is not limited by one generation. However the consequences of acute stress happened in early period of gestation are rarely investigated. So the aim of the present study was to evaluate acute hypobaric hypoxia effects on pregnant rats (generation F0) as well as on their direct descendants (generation F1) and descendants resulted by interbreeding females from generation F1 with intact males (generation F2). Methods: The white rats on the 9−10th days of gestation were subjected to acute hypobaric hypoxia in an altitude chamber. The first day of pregnancy was determined by the presence of spermatozoa in the vaginal smear. Control pregnant females were not subjected to acute hypobaric hypoxia. On the 2nd day after the delivery pups were weighed, and from the 3rd till the 8th day post partum maternal behavior in the home cage was observed. A modified ‘snap-shot’ method of Myers has been used: every rat has been observed for 5 seconds every 5 minutes. A number of variables such as mother being in or out of nest, being in contact with pups, licking/grooming of pups, nursing, etc. has been recorded. After growing up the female posterity (F1 dams) were mated with intact males, and their maternal behavior was observed in the same way. Two posterity generations has been grown up. The estimation of physical development in pups was carried out from the day of birth up to the 57th day of life. At the age of 22 (critical age for survival because of the end of weaning and switch to adult nutrition type) and 57 days (considered to be pubertal period) the motor and exploratory activity of experimental animals were tested in “Hole board” while the anxiety level was assessed using an elevated plus-maze. The cognitive function was tested in complex maze with positive reinforcement. Results: It was shown that pregnant rats survived hypoxia (F0) demonstrated the increased maternal behavior (more contact with pups and total nursing then control dams) while females from generation F1 showed its decrease (more leavings of nest, less contact with pups and less total nursing). Despite of oppositely directed modifications of maternal behavior, demonstrated by F0 and F1 females, F1 and F2 posterity showed similar deviations of physical development and behavioral. Acute hypobaric hypoxia led to the delay in eyes opening and the decrease of height and weight in posterity of F1 and F2 generations, compared to control animals. Besides males and females of both generations demonstrated the decreased linear locomotion and exploratory activity following with raising in anxiety level. At the same time the learning capacity was improved in females from F1 generation and was reduced in F2 males. Conclusion: Data obtained support the great role of early organogenesis for development of not only direct posterity but also following generations. [ DOI ]

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