Surface modification with polyallylamines for immobilization of biopolymers and cellsтезисы доклада

Информация о цитировании статьи получена из Web of Science
Дата последнего поиска статьи во внешних источниках: 3 июля 2019 г.

Работа с тезисами доклада


[1] Surface modification with polyallylamines for immobilization of biopolymers and cells / N. Barinov, O. Morozova, O. Levchenko et al. // FEBS Open Bio. — Vol. 8 of Supplement 1. — FEBS Open Bio Prague, Czech Republic, 2018. — P. 475–475. Surface modification for biomedical, environmental, agricultural and security applications can be performed physically and chemically by surface coverage or grafting with polymers and conjugating ligands. The immobilized substances must retain their structures and not desorb from the functionalized surfaces. Hydrophobic, electrostatic and vanderWaals interactions, coordination and covalent binding are involved in the adsorption. Our aim was the development of the functionalization method for immobilization of native proteins and viable cells. Cationic polymers with NH2groups were used for functionalization of charged and uncharged surfaces of planar slides, wells of plates and spherical nanoparticles. Poly(allylamine)s (PAA) and polylysines (pLys) of different molecular weights formed interfaces of the thicknesses ∼1.52 nm for PAA 65 kDa as measured by: 1) the atomic force microscopy on mica slides; 2) the labelfree biosensor with registration of the critical angle of total internal reflection on photonic crystal surface covered with SiO2. Sorption capacity of 0.1 mg/ml PAA 65 kDa exceeded the values of other polyamines with different concentrations. Physisorption of proteins on PAAlayer is reversible and up to 70% of attached proteins can be removed. Glutaraldehyde provides stable chemical crosslinking of the compounds containing primary NH2groups with the aminated surfaces. The proteins immobilized on the pAAcovered surface retained their ability to bind with specific monoclonal and polyclonal antibodies. Bacterial cells attached to PAA65covered surfaces could express the green fluorescent protein (GFP) gene under control of the inducible lac promoter. Eukaryotic cells also remained alive on PAAtreated slides as shown by fluorescent staining. Thus, treatment of plain and spherical surfaces with PAA is convenient for attachment of biopolymers and viable cells. The authors acknowledge funding from the Russian Science Foundation [177530064 to D.V.K.].

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